JEOL 1400 Cryo-TEM
This microscope is designed for both 2D and 3D imaging and characterization of soft materials in a near-native state, achieved by rapid freezing under liquid nitrogen temperatures for cryo-electron microscopy (cryo-EM). When combined with the in-house–developed Individual-Particle Electron Tomography (IPET) reconstruction method—further enhanced by machine learning techniques to improve contrast in low-dose images—the instrument is particularly well-suited for non-averaged 3D reconstruction of individual biomolecular particles at nanometer resolution. This capability enables statistical analysis of continuous conformational changes and real-time tracking of macromolecular dynamics during chemical reactions.
In addition, the system supports micro-electron diffraction (MicroED) for intermediate-resolution structural determination. It is a 120 kV LaB₆ filament transmission electron microscope (TEM), equipped with a high-sensitivity Gatan OneView camera optimized for low-dose imaging. The system is also compatible with negatively stained samples, making it ideal for the initial screening of soft and biological specimens.
For suitable samples requiring high-resolution cryo-EM imaging and 3D reconstruction, the UC Berkeley Titan Krios G3 equipped with a Gatan K3 direct electron detector will serve as a complementary platform.
